Our chambers carry a variety of critical features for tissue growth and 3-D culture.
Our bioreactor chambers are third generation designs, and capture the benefits of customer feedback. Chambers can be tailored to grow/culture different tissues such as: blood vessels, ligaments, cartilage and bone by stimulating cell-seeded scaffolds via perfusion or controlled mechanical deformation. Though each has unique features, all retain common design characteristics required to make your experiments productive:
The LumeGen V60 Series of vascular chambers facilitate pulsatile and shear stress stimulation to a single vascular construct. Standard construct sizes may range from 2 - 6 mm inner diameter and from 10 - 60 mm long. The modular design allows installation of the scaffold and cell seeding independently of the stimulation system. Multiple ports are available for media access, catheter probe insertion, sample collection, or further customization. The chamber has a media volume of 60mL.
| Chamber | Sample Number | Max Sample Size | Chamber Volume |
|---|---|---|---|
| V60-1r | 1 | 60mm long x 6mm ID | 60ml |
The LigaGen L30 Series of Chambers delivers oscillatory compressive/tensile axial stimulation to samples with a large aspect ratio. The chambers can be used with a variety of construct materials from decellularized tendons and ligaments to polymerized hydrogels. The chamber accommodates samples up to 30 mm long, and 3 mm thick. Multiple grip styles allow for a wide range of construct geometries to be stimulated. A unique seal proves a mechanical feed-through into the chamber, while facilitating axial motion in a sterile environment with minimal resistance. Two different chamber designs allow either single sample or multi-sample stimulation. Both chambers can be used with a perfusion system to provide convective media transport around the sample.
| Chamber | Sample Number | Max Sample Size | Chamber Volume |
|---|---|---|---|
| L30-1X | 1 | 30mm long x 3mm thick | 23 ml |
| L30-4C | 2 or 4 | 30mm long x 3mm thick | 80 ml |
| L150-1 | 1 | 150mm long x 5mm thick | ~75ml |
| Grip | Chamber Availability | Features |
|---|---|---|
| 40N Spring Assisted Wedge Action Grip |
L30-1X, L30-4C | 4.8mm wide wedge shaped grip faces with gentle convolutions designed to secure slippery tissue. Accommodates samples 3mm thick and 4mm wide. |
| 40N Hydrogel Grips |
L30-1X | Zero-clamp design includes an integrated construct mold to enable mechanical stimulation of hydrogel scaffolds |
| 40N Screw Action Grips |
L30-1X, L150-1 | Flat faces with serrated surfaces, provide a simple and efficient method to secure biological tissue. Available in 6mm wide or 10mm wide configurations. |
| Custom Grips | L30-1X, L30-4C | Custom designed for specific scaffold textures or geometries |
The CartiGen C10 Series of Chambers delivers oscilatory compressive stimulation4 to disc shaped samples. The chambers can be used with a variety of construct geometries by choosing one of our many sample platen options, maximum sample size per individual well is 10 mm diameter by 5 mm thick. The maximum sample size for single sample chamber is 30 mm diameter by 5 mm thick. A unique seal provides a mechanical feed-through into the chamber while facilitating axial motion in a sterile environment while allowing axial motion with minimal resistance. Perfusion through a single construct is available with porous platens. The C10-12c chamber has optical grade windows on the bottom, allowing researchers to visualize their samples in real time, via confocal microscopy, without interrupting the culture.
| Chamber | Sample Number | Max Sample Size | Chamber Volume |
|---|---|---|---|
| C10-1 | 1 | 30mm diameter x 5mm thick | 10 ml |
| C10-12c | max 12 | 10mm diameter x 5mm thick | 2ml/well |
| Platen | Chamber Availability | Features |
|---|---|---|
| Smooth | C10-1, C10-12c | Autoclavable polypropylene, smooth flat face construction. |
| Porous | C10-1, C10-12c | Sintered stainless steel. Enables perfusion through the platen and into construct bottom face. |
| Hydrogel | C10-1, C10-12c | Porous polymer design allows the sample to be cast into platens, preventing the gel from moving. |
| Custom | C10-1, C10-12c | A modified version of BISS's standard platen, built to your specification. |
The OsteoGen O10 Series of Chambers deliver perfusion5 and hydrostatic pressures to a cell seeded cylindrical scaffold. The chamber accommodates samples up to 10 mm diameter and 10 mm long. The translucent polymer housing allows visualization of the construct and media during culture.
| Chamber | Sample Number | Max Sample Size | Chamber Volume |
|---|---|---|---|
| O10-1 | 1 | 10mm diameter x 10mm long | 2 ml |
The DermiGen D70-1 Series of chambers delivers oscillatory tensile axial stimulation to samples with a large aspect ratio. The chambers can be used with a variety of construct materials. The chamber accommodates samples up to 70 mm long, 20mm wide, and 2 mm thick. The chamber body's unique design provides an air/media interface. The chamber assists media perfusion/flow along the underside of the construct while deterring media from spilling onto the topside. Custom mechanical grips with a soft lining provide secure positioning of the sample. A unique seal provides a mechanical feed-through into the chamber, while facilitating axial motion in a sterile environment with minimal resistance.
| Chamber | Sample Number | Max Sample Size | Chamber Volume* |
|---|---|---|---|
| D70-1 | 1 | 70mm long x 20mm wide x 2mm thick | 15 ml |
*Chamber volume reported assumes chamber is being used with air/media interface
